Captain America Steve Rogers (Chris Evans), who is gradually accustomed to modern life, vaguely smells the dangerous smell that pervades S.H.I.E.L.D. after an operation. When he learned that S.H.I.E.L.D. was secretly conducting the "Insight Project", he was even more angry about it. One day, S.H.I.E.L.D. commander Nick Fari (Samuel L. Jackson) is attacked by a group of militants. He tries his best to hand a USB flash drive to Captain America, which hides the bureau's most important and terrifying secrets since World War II. After that, Captain America was wanted and chased by former police chief Alexander Pierce (Robert Redford/Robert Redford) on suspicion of killing Nick. In the escape, he was helped by the Black Widow (Scarlett Johansson) and veteran "Falcon"(Anthony Mackie). They must race against time to unlock the secrets of S.H.I.E.L.D. and prevent the implementation of Project Insight...